Microsatellite Genotyping From CD Genomics Can Be Used For The Analysis Of Transmission Characteristics In Genital Candida Albicans Infection

Candida albicans is the most common pathogenic bacteria in superficial and systemic candidiasis. Because the traditional identification technology is difficult to identify strains, restricting the study of the epidemic characteristics and infection characteristics of Candida albicans.

This study is based on the microsatellite polymorphic sequence characteristics of cell division cycle protein gene, elongation factor 3 gene and imidazole glycerol phosphate deshydratase gene, to establish improved microsatellite genotyping method for strain identification, aimed at detecting the genotypes of the main pathogen infected by Candida albicans and their possible sources, and exploring the propagation characteristics of Candida albicans.

Candida albicans is the main pathogenic fungi of genital candidiasis. It is cultured by the traditional method based on the cell phenotype characteristics, which has very important value for distinguishing beads. With the emergence of genotyping technology, the detection method based on the type of system can significantly improve the discrimination ability of different strains.

Genotyping technology of Candida albicans by microsatellite DNA sequence polymorphism has high identification ability and strong specificity. Candida albicans diploid microsatellite polymorphism is decided by three aspects: allelic polymorphism; two alleles composed gene polymorphism; polymorphism of multiple microsatellite combined analysis, which determine the high discriminatory ability of microsatellite genotyping.

Single microsatellite sequence polymorphism typing ability reaches 70%-80%, while three microsatellite markers could reach 97%. But diploid characteristics in C.albicans genome make different microsatellite two alleles difficult to distinguish. Microsatellite sequences cloned sequences cannot ensure to get two allelic gene cloning, and the method is cumbersome and expensive.

Three gene analysis shows that 116:124, 122:131, 160:200 are the main genotypes for pathogenic Candida albicans, suggesting that some special microsatellite genotype plays a dominant role in the infection of Candida albicans. These genotypes has important role as biomarkers for further study of pathogenic strains toxicity and pathogenicity.

High detection rate of female genital Candida albicans to infect anal canal and high consistency of genital and anal Candida albicans genotyping suggest that intestinal Candida albicans may be the important endogenous sexy infection sources of female genital candidiasis. And male genital Candida albicans rate is very low. Only 3.8% of the patients have the same Candida albicans genotypes in genital and anal canal, suggesting that male genital Candida albicans infection pathogenic strains may not mainly derived from the anal canal. Genital infection rate of male and female patients is very low. Vaginal and oral Candida albicans genotypes are less consistent, suggesting that oral Candida albicans is not the main source of genital infection.

In conclusion, the improved Candida albicans microsatellite genotyping technique is important for clinical isolation, tracking of infection sources, route of transmission and discovery of susceptibility genes.

Contact Info:
Name: Debbie Evans
Email: Send Email
Organization: CD Genomics
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA
Phone: 5166698109
Website: http://www.cd-genomics.com/

Release ID: 119572