Microbiosci, the major division of Creative Biogene, is devoted to providing global scientists with high quality products and satisfactory services to facilitate the investigation of microbial researches. With the accumulated experience and professional team, Microbiosci announced the release of its diverse microbial cell-surface display service in order to accelerate the research in the target protein or peptide on the surface of bacteria via genetic fusion to anchoring motif. —
The microbial cell surface display technology offered by Microbiosci has considerable advantages:
• Maintain the relatively independent spatial structure and biological activity of the protein
• Protein is more active and stable
• Greatly simplified the protein preparation process
• Reduced production costs
Microbiosci knows that yeast display technology has been used in a variety of biotechnological and biomedical applications, such as electrochemical equipment, biocatalysis, biosorption and bioremediation, enhanced resistance to organic solvents, protein-protein and protein-ligand Interactions, vaccine production, antibody engineering, biosensor design, bioethanol production. Microbiosci utilizes a variety of analytical verification methods, including SDS-PAGE analysis, Western blot analysis, enzymatic determination, fluorescence determination, flow cytometry, and immunofluorescence microscope to provide stable, efficient and successful yeast display technology for industrial-scale recombinant protein production.
In recent years, considerable momentum has been gained by displaying heterologous proteins or peptides on the surface of bacteria. With the availability of a variety of well-proven protein expression and secretion systems, E. coli is a more suitable microorganism. Studies have shown that the applications of bacterial display include but are not limited to: live vaccine development, antibody production, peptide library screening, environmental biosorbents, whole cell biocatalysis. Microbiosci provides E. coli surface display services to display foreign proteins or peptides realized through recombinant DNA technology. With a wealth of experience, Microbiosci's talented scientists have provided appropriate construction strategy recommendations for various biotechnology applications.
In the past few decades, Lactic Acid Bacteria (LAB) has been very important for applications in the food, beverage and feed industries. In addition, lactic acid bacteria cell surface display is currently a promising technology that delivers active molecules such as vaccines or cytokines to mucosal tissues. Compared with cytoplasmic expression or secretion, the cell wall-anchored E7 produced on the surface of LAB triggers a better antigen-specific cellular response. Because of this potential, the improvement of genetic tools for effective protein expression, secretion and cell surface display in lactococcus and lactobacilli has attracted more and more attention. Microbiosci has developed various strategies based on anchoring proteins to the surface of LAB, providing surface display services of Lactococcus lactis to display functional proteins on the surface of LAB or deliver active molecules to mucosal tissues.
“Generally, the turnaround time for our microbial surface display service is 4-8 weeks, depending on the scale of your project.” said Marcia Brady, the marketing director of Creative Biogene, she also added, “We provide a variety of cell surface display systems that can successfully display target proteins or peptides on the surface of bacteria through gene fusion with anchor motifs. Microbial genome editing technology can also be used to produce sandwich fusions for further constitutive expression and surface display.”
Microbiosci, as the division of Creative Biogene, is always dedicated to satisfying the needs of clients covering more than 50 countries and districts. As a leading custom service provider in delivering medicine microbiology solutions, Microbiosci has become a well-recognized industry leader with years of experience and professional scientists.
Release ID: 88983285