Microbiosci, the mature division of Creative Biogene, is fully engaged in providing high quality products and servives related to microbiology in order to facilitate the investigation of microbial research. Recently, Microbiosci announced the release of its cell surface display system for the successful display of a target protein or peptide on the surface of bacteria via genetic fusion to anchoring motif. —
Microbial cell surface display technology has great strategic potential in expressing heterologous proteins or peptides with unique functions on the surface of microbial cells. In order to obtain suitable cell surface exposure, passenger proteins can be fused with various anchoring motifs, which are usually cell surface proteins or fragments thereof. According to the characteristics of the passenger and carrier protein, different fusion methods can be used, including C-terminal fusion, N-terminal fusion or sandwich fusion to implement this strategy.
Microbial cell surface display technology has considerable advantages. It can maintain the relatively independent spatial structure and biological activity of the protein. The protein is more active and stable, which greatly simplifies the protein preparation process and reduces production costs. Creative Biogene has many years of service experience in cell surface display and has a world-class service platform. One-stop service can ensure efficient service for customers, and can provide emergency services with regular progress updates.
Creative Biogene provides a variety of cell surface display systems that can successfully display target proteins or peptides on the surface of bacteria by fusing genes to anchor motifs. Microbial genome editing technology can also be used to produce sandwich fusions for further constitutive expression and surface display. Meanwhile, Creative Biogene can verify epitopes through SDS-PAGE, Western blotting, flow cytometry and immunoelectron microscopy of intact bacteria.
Currently, Creative Biogene has developed a recombination method based on the modification of the Bacillus subtilis genome to show that the passenger protein of interest is fused with the spore coat protein. During the experiment, the non-essential genes amyE, lacA and pyrD were selected as insertion sites to create a multi-anchored protein display system. With a wealth of experience, Creative Biogene's talented scientists provide appropriate construction strategy recommendations for various biotechnology applications.
“Besides, the other cell surface display services available at Microbiosci include E.coli surface display service, L. lactis surface display service and yeast cell-surface display service,” said Marcia Brady, the marketing director of Creative Biogene, she also added, “Our experienced scientists and professional platform support the team to customize unique solutions if our customers desire to apply a specific plan for the research.”
Microbiosci, as the division of Creative Biogene, is always dedicated to satisfying the needs of clients covering more than 50 countries and districts. As a leading custom service provider in delivering medicine microbiology solutions, Microbiosci has become a well-recognized industry leader with years of experience and professional scientists.
Name: Marcia Brady
Email: Send Email
Organization: Creative Biogene
Address: Shirley, NY 11967, USA
Release ID: 89003530