-- Creative Proteomics announced the launch of a Glycolysis Pathway Analysis platform that incorporates LC-MS/MS metabolomics, stable isotope tracing, and computational flux modeling for a complete understanding of dynamics within central carbon metabolism.
The platform utilizes ultra-high resolution Orbitrap mass spectrometry and hydrophilic interaction liquid chromatography (HILIC) to achieve femtomolar sensitivity for major intermediates of glycolysis, including glucose-6-phosphate, fructose-1,6-bisphosphate, and phosphoenolpyruvate. The technical advances made by Creative Proteomics allow researchers to identify glycolytic flux modulations that are not detected by traditional techniques.
"Our new platform is unique because we have integrated targeted quantitative proteomics of rate-limiting glycolytic enzymes such as hexokinase, phosphofructokinase, and pyruvate kinase with comprehensive metabolite profiling," said the Technical Director at Creative Proteomics. "We provide a full systems-level view of glycolytic regulation by simultaneously monitoring enzyme expression, post-translational changes, and abundance of glycolytic metabolites, therefore providing a level of understanding that goes beyond traditional metabolomics."
The services include the ability to use 13C-glucose tracing to distinguish glycolytic contributions from the pentose phosphate pathway and TCA cycle to cellular energetics. By tracing isotopomers and quantifying responses, the test also identifies patterns of metabolic rewiring known to occur in cancer driven by the Warburg effect or other disease states.
Key technical specifications and capabilities of the platform include:
- Quantitative analyses of 27 metabolites related to glycolysis with >95% coverage
- Integrated proteomics analysis of 14 key regulatory enzymes
- Detection and derivation of flux from stable isotope studies with 13C, 15N, and 2H
- Computational modeling using state-of-the-art kinetic frameworks
- Custom designed inhibitor screening for glycolytic enzymes
- Compatibility with a variety of sample types, e.g., cell cultures, tissues, biofluids
- Protocols capable of studying cells under hypoxic and nutrient-deprived conditions
"The platform has already, and is continuing to provide an opportunity for oncology researchers studying cancer metabolic vulnerabilities in tumors with dysregulated PI3K/AKT/mTOR signaling pathways," said the Technical Director. "Creative Proteomics has worked with over a dozen pharmaceutical clients to identify new targets in glycolysis-dependent cancers and validate mechanism-of-action for drugs targeting metabolic enzymes like PKM2 and LDHA."
The platform and service includes sophisticated bioinformatics analysis and experimental frameworks that combine pathway enrichment algorithms with KEGG database integration and machine-learning-based approaches to identify bottlenecks and regulatory nodes within glycolytic metabolic networks.
Creative Proteomics now offers customized experimental designs for researchers studying reversal of the Warburg effect, mitochondrial dysfunction, and metabolic reprogramming, among other applications.
About Creative Proteomics
Creative Proteomics provides state-of-the-art services that combine metabolomics, proteomics and bioinformatics to biomedically accelerate research and the discovery of therapeutics that treat metabolic disorders, cancer, and rare diseases.
Contact Info:
Name: Dora West
Email: Send Email
Organization: Creative Proteomics
Website: https://www.creative-proteomics.com/
Release ID: 89158839
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