With decades of experience in supporting researchers with all kinds of high-quality services and products to explore questions about gene discovery, regulation and so on, Creative Biogene continues to develop much more advanced techniques like — Tn5 transposase.
Robust Tn5 transposase, belonging to the RNase superfamily of proteins, can be found in Shewanella and Escherichia bacteria. The Tn5 transposon codes are for antibiotic resistance to kanamycin and other aminoglycoside antibiotics.
Because of the inherently mutagenic DNA transposition events, Tn5 and other transposases are notably inactive, which is playing a crucial role in reducing the risk of causing a fatal mutation in the host, eliminating the transposable element. And due to this inactive features, robust Tn5 transposase in Creative Biogene can be used to recognize a series of end sequences, including inside end sequences, outside end sequences and mosaic end sequences of Tn5 transposon.
Tn5 and most other transposases contain a DDE motif, which is the active site that can catalyze the movement of the transposon. It is also said to coordinate divalent metal ions, most often magnesium and manganese. Because of the inactive features, the DDE region is mutated, which allows the transposase becomes hyperactive and catalyzes the movement of the transposon.
“the research of rubost Tn5 transposase has become more and more important and practical in Creative Biogene. Because it has similarities to HIV-1 and other retroviral diseases.” said Dr. Alexander, a senior scientist in Creative Biogene, “as well as this, Tn5 in Creative Biogen is also applied in recognition of various end sequences and genome sequencing for fragmentation of the DNA in the technique, which is called ATAC-seq.”
About Creative Biogene
Creative Biogene is an outstanding manufacturer and provider of genomics and proteomics related products and services for research and academic use. Business scope ranges from basic laboratory research to biotechnological industries. And now the rubost Tn5 transposases service has been applied by scientists all over the world.
Release ID: 123012