Amerigo Scientific, a distribution company focused on providing critical products and services to the biomedical and life science research communities, now introduces — Recombinant PNGase F that can be used for various research applications such as glycan release.
PNGase F cleaves asparagine-linked (N-linked) oligosaccharides from glycoproteins. PNGase F deaminates asparagine to aspartic acid, leaving the oligosaccharides intact. Denaturation increases the rate of cleavage up to 100x. Most native proteins can still be completely N-deglycosylated but incubation time must be increased. PNGase F will remain active under incubation conditions for at least 72 hours. PNGase F will not remove oligosaccharides containing α(1,3)-linked core fucose commonly found on plant glycoproteins; for this purpose, you can use peptide N-glycosidase A.
PNGase F is suitable for release of all types (high-mannose, hybrid and complex) N-linked glycans from glycoproteins and glycopeptides. PNGase F will not remove oligosaccharides containing α(1-3) linked core fucose commonly found on plant glycoproteins. Amerigo Scientific now offers new Recombinant PNGase F that is isolated from a E. coli strain containing a clone of the Elizabethkingia miricola gene. There is no detectable difference in activity or specific activity of the recombinant enzyme from the native enzyme (E-PNG01).
Amerigo Scientific offers a comprehensive collection of enzymes from global manufacturers covering all the core biological research areas. Enzymes are biological catalysts that regulate the rate of chemical reactions that proceed in organisms and play irreplaceable roles in signal transduction pathways and cell regulation pathways. This new Recombinant PNGase F is an addition of the the enzymes products.
"Life science research expands our scientific understanding and help discover more findings to meet unmet medical needs. As a life science distribution company, Amerigo Scientific never forgets providing quality enzymes products to the research communities. We're glad to expand our portfolio, and we'll continue to improve our ability to enable us to continue to deliver innovative solutions to our customers." said Nina Cooper, Ph.D., the chief scientist at Amerigo Scientific. "This Recombinant PNGase F product is intended for in vitro research only. And it is not intended for human or animal diagnostic or therapeutic uses or uses in the manufacture of food or pharmaceutical products." added Nina.
For more information about the Recombinant PNGase F, please visit https://www.amerigoscientific.com/.
About Amerigo Scientific
Amerigo Scientific is a distribution company that focuses on providing critical products and services to the biomedical and life science research communities. Although it is a new company, Amerigo Scientific's founder has had more than 20 years of rich experience in the biomedical and biochemical fields, and has established close contacts with key personnel in top international pharmaceutical and biotech companies, academic research institutes, and government research agencies. It has a professional team, as most of its employees own a graduate (Ph.D. or master) degree in life science, so they can understand customers' questions or concerns and are always ready to provide individualized customer service with high standard.
Release ID: 89043677