-- As regular gut microbiota, Bacteroides spp. are among the most researched gut microbiota owing to their immune system modulation and metabolism. Over the years, the company has devoted considerable resources to functional strain research on the Bacteroides spp. and functionally innovative experimental design.
Precision Control and Safety Evaluation: Bacteroides fragilis
Bacteroides fragilis is an anaerobic bacterium with both commensal and potentially pathogenic properties, and its capsular polysaccharide is thought to be critical to inducing immune tolerance and maintaining mucosal homeostasis.
Creative Biolabs' Bacteroides fragilis CRO services implement a rigorous screening and safety evaluation workflow, including strain isolation under controlled oxygen conditions, bft gene PCR testing, MALDI-TOF analysis, and whole-genome sequencing to ensure functional activity and safety of research strains.
This service encompasses all aspects of fed-batch fermentation, down to the quantification of metabolites, so that researchers can assess SCFA production, control carbon flux, and manage stress response. Mechanistically, the team works collaboratively on immune co-culture systems and pathway assays to study PSA-induced immunomodulation and ETBF-induced epithelial signaling, as they develop primary materials relevant to study the immune system's mucosa and inflammatory diseases.
Polysaccharide Metabolism and Host Signaling: Bacteroides thetaiotaomicron
Bacteroides thetaiotaomicron is a prominent polysaccharide degrader in the gut, known for extensive polysaccharide utilization loci (PULs) and a complex metabolic network.
Creative Biolabs' Bacteroides thetaiotaomicron services provide an efficient way to assess its functional roles in nutrient flux, immune signaling, and barrier homeostasis. Services include:
* Strain screening and identification: High-activity strains are recovered from biological samples using selective bile-esculin agar (BBE) and targeted enrichment, followed by precise identification through 16S rRNA and whole-genome sequencing.
* Metabolic profiling: Both polysaccharide fermentation capacity and SCFA fingerprinting are assessed under controlled, low-oxygen conditions to identify substrate preferences.
* Host interaction studies: Co-culture systems with epithelial cells or organoids evaluate regulation of tight junction proteins, mucus layer dynamics, and cytokine expression.
* Immune response assessment: Human PBMCs and dendritic cell models are used to assess the effects of B. thetaiotaomicron and its metabolites on IL-10, IL-6, TNF-α, and other immune profiles.
Bacteroides vulgatus Case Study
Creative Biolabs Bacteroides vulgatus CRO services provide a complete end-to-end service from strain characterization to formulation proof of concept, including fermentation process optimization, lyoprotectant screening, stability testing, and functionality testing.
In a representative example, a freeze-dried B. vulgatus powder, which featured a cryoprotectant system designed for the probiotic, had 5 × 10⁸ CFU/mL after rehydration to provide assurance of activity. Studies have shown that various combinations of cryoprotectants (trehalose and skim milk) have been identified and subsequently developed into significantly better formulations with respect to recovery and membrane integrity.
This allows for an anaerobic probiotic to be handled and delivered under controlled conditions with active survival throughout storage and transport over time. This demonstrates Creative Biolabs has viable methodologies in place to control the formulation and quality control assurance of an anaerobic probiotic.
About
Creative Biolabs Bacteroides spp. service platform and CRO service provide the research community with high standards of traceability and evidence-based support, bridging the gap between microbiome and translational applications.
Contact Info:
Name: Candy Swift
Email: Send Email
Organization: Creative Biolabs
Website: https://live-biotherapeutic.creative-biolabs.com/
Release ID: 89173064
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