The identification of gene function is a fundamental goal of functional genomics, with scientists aiming to link genotype to phenotype to determine cellular processes, understand the causative agents of disease, and identify targets for drug discovery. Providing a better means to explore the secret of gene, CRISPR technology has rapidly changed the biological research, such that precise genome editing has now become routine for many labs within several years of its initial development. —
With more than a decade of exploration and expansion, Creative Biogene is confident to offer the one-stop CRISPR library screening services for global customers, including CRISPR knockout screening, CRISPRi screening and CRISPRa screening.
CRISPR Knockout Screening
As a useful approach to identify genes essential for different biological processes, CRISPR knockout screening helps to uncover new roles of known genes. Combining with different compounds, this screening is efficient to find potential targets for the new drug.
CRISPRi screening represses expression rather than completely knocking out the target gene and to evaluate the function of genes that when knocked-out is essential or that is amplified.
CRISPRa screening amplifies gene expression in its endogenous context and enables for the first time, to study activation-linked responses on a genome-wide level. This screening, which helps to study the functions of genes that express low in natural condition, is the only method capable of genome-wide functional gain screening, capable of identifying endogenous genes that change phenotype when activated rather than destroyed.
Scientists in Creative Biogene have extensive experience in custom screening design with access to cell lines from pre-optimized cell series tables or cell line assessments before screening. Targeted gene CRISPR lentivirus library for targeted multi-species genome/transcriptome screening libraries, including human, mouse, rat, zebrafish, nematode, fruit fly, etc. is also available. Besides, Creative Biogene provides dual sgRNA library construction-contains two enhanced activations and inhibitions for each gene 5 sgRNAs designed for the promoter region of each gene for optimal inhibition or activation.
“Our CRISPRi/CRISPRa screening performs well in assessing the effects of activating or inhibiting thousands of different genes on cell growth or other screenable phenotypes.” Introduced by a senior scientist in Creative Biogene, “it also helps to identify potential therapeutic targets which are responsible for controlling growth and differentiation, regulating disease development or other biological responses.”
CRISPR is a useful tool for gene screening experiments because it is relatively easy to design gRNA and has the ability to modify almost any gene locus. Creative Biogene CRISPR/Cas9 Platform provides cover-all CRISPR-Cas9 services and products from sgRNA design through transfection and selection to final product verification and sequencing. To date, Creative Biogene has accumulated extensive experience from its success in lots of CRISPR-Cas9-related projects. Further information can be found on https://www.creative-biogene.com/crispr-cas9/.
About Creative Biogene
With an unrelenting drive in bio-scientific research and industry, Creative Biogene offers innovative technologies, products, unique tools and services for research discoveries and product development. CRISPR/Cas9 Platform, a division of Creative Biogene, has developed all-inclusive customized gene-editing services as well as products for clients worldwide.
Release ID: 88943546